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R 9 is cyano, halogen (eg, fluorine, bromine, chlorine, iodo, etc), nitro, hydroxyl, alkoxy, or any other substituent which allows the tetracycline compound to perform its intended function. The main multi-center study, sponsored by the manufacturer, showed Neurontin slightly less effective than placebo, and the results were not published for quite a while!
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level of expression of a gene encoding a particular revTetR protein may be manipulated by the choice of promoters with different transcription rates to which the revTetR coding sequence is operably associated, the inclusion of one or more positive and/or negative regulatory sequences which control the rate of transcription from that promoter, and the copy number of the vector carrying the revTetR coding sequence. Do not take a double dose to make up for a missed one?
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